Conidiating colonial strains suitable for replication

Isogenic Strains of PHYCOMYCES BLAKESLEEANUS Suitable for Genetic Analysis.

The progeny of crosses between wild-type strains of Phycomyces usually do not exhibit all of the expected genotypes from meiosis. By backcrossing, we have isolated a new (+) mating-type strain, A56, which is nearly isogenic with the (-) wild-type NRRL1555 commonly used in Phycomyces research. Tetrad analysis of the backcrosses shows that meiosis becomes more regular as the parental (+) and (-) ...

Attenuated and replication competent Vaccinia Virus strains

16 Replication competent poxvirus vectors with an attenuation phenotype and with high 17 immunogenic capacity of the foreign expressed antigen are being pursuit as novel 18 vaccine vectors against different pathogens. In this investigation we have examined the 19 replication and immunogenic characteristics of two vaccinia virus (VACV) mutants 20 M65 and M101. These mutants were generated after ...

Role of Interferon in Replication of Virulent and Attenuated Strains of Measles Virus

A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains.

AIMS A simple and rapid method (designated thermolysis) for extracting genomic DNA from bulk fungal strains was described. METHODS AND RESULTS In the thermolysis method, a few mycelia or yeast cells were first rinsed with pure water to remove potential PCR inhibitors and then incubated in a lysis buffer at 85 degrees C to break down cell walls and membranes. This method was used to extract ge...

Screening of Bacterial Cellulose - producing Acetobaeter Strains Suitable for Agitated Culture

particularly eMcient. The cellulose productiyities of 412 isolates were estimated by cultuTing in two different media under both shaken and static conditions. No correlation between the amounts of cellulose accumulated in shaken and static cultures was observed. Higher cellulose accumlllation was obtained in the shaken cultures using a corn steep liquorlfructose-based medium than a conyentional...